Caspase-3 Cleavage and Nuclear Localization of Caspase-Activated DNase in Human Temporal Lobe Epilepsy
نویسندگان
چکیده
منابع مشابه
Molecular cloning and characterization of human caspase-activated DNase.
Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Here, we report isolation of two classes of human CAD cDNAs from a human KT-3 leukemic cell cDNA library. One class of cDNA encoded a protein comprising 338 amino acids, which showed a marked similarity to its murine counterpart. In vitro transcription and translation of this cDNA resulted in a functional CAD protein when t...
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Caspase 3 is required for the differentiation of a wide variety of cell types, yet it remains unclear how this apoptotic protein could promote such a cell-fate decision. Caspase signals often result in the activation of the specific nuclease caspase-activated DNase (CAD), suggesting that cell differentiation may be dependent on a CAD-mediated modification in chromatin structure. In this study, ...
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We show that Cdc6, an essential initiation factor for DNA replication, undergoes caspase-3-mediated cleavage in the early stages of apoptosis in HeLa cells and SK-HEP-1 cells induced by etoposide, paclitaxel, ginsenoside Rh2, or tumor necrosis factor-related apoptosis-inducing ligand. The cleavage occurs at the SEVD442/G motif and generates an N-terminal truncated Cdc6 fragment (p49-tCdc6) that...
متن کاملContrasting nuclear dynamics of the caspase-activated DNase (CAD) in dividing and apoptotic cells
Although compelling evidence supports the central role of caspase-activated DNase (CAD) in oligonucleosomal DNA fragmentation in apoptotic nuclei, the regulation of CAD activity remains elusive in vivo. We used fluorescence photobleaching and biochemical techniques to investigate the molecular dynamics of CAD. The CAD-GFP fusion protein complexed with its inhibitor (ICAD) was as mobile as nucle...
متن کاملP8: Temporal Lobe Epilepsy and Mitochondrial Dysfunction
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ژورنال
عنوان ژورنال: Journal of Cerebral Blood Flow & Metabolism
سال: 2005
ISSN: 0271-678X,1559-7016
DOI: 10.1038/sj.jcbfm.9600219